Differences between Zheng and Zhao (2022) and Roginski et al. (2024): DeNovoGeneAnnotation: inputData: values in Zheng and Zhao (2022) but not in Roginski et al. (2024): transcriptome inputTranscriptome: values in Zheng and Zhao (2022) but not in Roginski et al. (2024): expressionLevel: 0.2 transContextChoice: intergenic antisense intronic overlap gene transORFChoice: custom choice customORF: all >=8aa non-canonical start >=20aa evolutionaryInformation: values in Roginski et al. (2024) but not in Zheng and Zhao (2022): selection: codeml taxonID: taxID: differing values in Zheng and Zhao (2022) and Roginski et al. (2024): Drosophila melanogaster-insects Drosophila melanogaster-drosophila homologyFilter: threshold: values in Zheng and Zhao (2022) but not in Roginski et al. (2024): unknown 0.0 values in Roginski et al. (2024) but not in Zheng and Zhao (2022): e-value 0.0001 dataBase: values in Zheng and Zhao (2022) but not in Roginski et al. (2024): custom choice values in Roginski et al. (2024) but not in Zheng and Zhao (2022): NCBI NR customDB: values in Zheng and Zhao (2022) but not in Roginski et al. (2024): Drosophila melanogaster proteome (FlyBase, r6.15) nonCodingHomologs: values in Roginski et al. (2024) but not in Zheng and Zhao (2022): enablingMutations: False synteny: anchors: gene anchors softwareSyntenySearch: DENSE translationalEvidence: values in Zheng and Zhao (2022) but not in Roginski et al. (2024): translationEvidence: mass spectrometry ribosome profiling studyURL: values in Zheng and Zhao (2022) but not in Roginski et al. (2024): https://doi.org/10.7554/eLife.78772 values in Roginski et al. (2024) but not in Zheng and Zhao (2022): https://doi.org/10.1093/gbe/evae159